Dry mass measurements in human diploid fibroblasts.
نویسندگان
چکیده
منابع مشابه
Senescent human diploid fibroblasts
The characteristic limited reproductive life-span of normal human fibroblasts in culture is due to a steadily decreasing fraction of cells able to proliferate in the standard rich growth media. We have observed that restricting the growth factor supply to old cells for variable lengths of time in culture increases the fraction of cells that can enter S-phase; although these cells do not go on t...
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Primary cultures of fibroblast-like cells were obtained from skin and articular cartilage of human donors in the age bracket of 1 to 15 years. For growth these cultures required 1 mg/liter of cholesterol added to Medium A2 plus acetyl choline, Na pyruvate, and D-galactosamine HCl (APG) containing 10% lipoprotein-free human serum. Established cell lines did not require cholesterol for growth. Ea...
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Two isoenzymes of ceramidase, including lysosomal acid ceramidase and nonlysosomal alkaline ceramidase, catalyze the degradation of ceramide in cultured human skin fibroblasts. A genetically determined disorder of sphingolipid metabolism (Farber's-disease) is characterized by the deficiency of acid ceramidase and by the pathological accumulation of ceramide. In this report, we take advantage of...
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Methionine restriction (MetR) extends lifespan in animal models including rodents. Using human diploid fibroblasts (HDF), we report here that MetR significantly extends their replicative lifespan, thereby postponing cellular senescence. MetR significantly decreased activity of mitochondrial complex IV and diminished the accumulation of reactive oxygen species. Lifespan extension was accompanied...
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Background & Aims: The aim of this study was to evaluate the accuracy of Cone-beam Computerized Tomography (CBCT) linear measurements in dry mandibles by comparing them with direct measurements. Methods: Two human dry mandibles were used in this study. Thirteen sites were selected on each mandible. Specimens were scanned by CBCT and linear measurements were made using Planmeca Romexis 2.3.0.R s...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1977
ISSN: 0022-1554,1551-5044
DOI: 10.1177/25.9.20472